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Fig. 3 | Biotechnology for Biofuels

Fig. 3

From: Mannanase hydrolysis of spruce galactoglucomannan focusing on the influence of acetylation on enzymatic mannan degradation

Fig. 3

Oligosaccharide product profiles obtained from HPAEC-PAD analysis of hydrolysis reactions (left panel), and quantification of M1–M4 oligosaccharides (right panel) after 24-h reactions with 100 nM CjMan5A or CjMan26A on KGMN (a, b), LBGN (c, d) and SpGGM (e, f). The profiles show that the two enzymes produced different amounts of the linear mannooligosaccharides M1–M4, as well as different types of complex oligosaccharides on the three substrates. M1 and M2 were the major mannans produced by CjMan26A, with a proportionally higher amount of M2 for the branched LBGN and SpGGM, while M1 was more dominant in the hydrolysis of KGMN. CjMan26A also generated a considerable amount of M3 on SpGGM. Hydrolysis with CjMan5 produced only small amounts of M1 and M2, while M3 and M4 were the main mannooligosaccharides produced. Duplicate measurements were performed, with standard errors of the mean of < 3%

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