Mannanase hydrolysis of spruce galactoglucomannan focusing on the influence of acetylation on enzymatic mannan degradation

Table 1 Compositions and molecular structures of the substrates used in this study

Sample	DSac^a	MW (kDa)	Carbohydrate composition %^b
Sample	DSac^a	MW (kDa)	Ara	Gal	Glc	Xyl	Man	Rha	GalA	GlcA
KGM_N	0.09	1000	0.1	0.5	38.9	0.2	60.3	–	–	–
KGM_A	0.7
KGM_B	2.1
LBG_N	0	1000	1.6	20.7	2.7	0.5	74.5	–	–	–
LBG_A	0.8
LBG_B	1.9
SpGGM	0.13	30	1.3	11.3	20.8	8.6	53.1	1.5	3.2	0.3

^a Degree of acetylation calculated by saponification and quantification of acetic acid using HPLC. Four individual measurements were made, with a standard deviation of < 10%
^b Carbohydrate composition (mol%), quantified with HPAEC-PAD. Duplicate measurements were made, with a standard deviation of < 7%, except for KGM Xyl, which had a standard deviation of 48%

ISSN: 2731-3654