Fig. 3

Plasmid copy numbers in Z. mobilis ZM4, 2032, and 8b under anaerobic conditions (a), aerobic conditions (b), and shift from aerobic to anaerobic conditions (c). All Z. mobilis strains were grown in RMG medium under anaerobic, aerobic or shift from aerobic-to-anaerobic conditions, and plasmid copy numbers were measured at both Mid-log and Stationary phases relative to the chromosome terminus assigned as 1 and corrected for PCR efficiency (see “Methods”). The chromosome ori copy number was determined as a control. Plasmid copy number measurements were based on at least three biological replicates, with the standard deviation indicated as error bar