Table 2 SNPs in Z. mobilis 2032 by comparing with new Z. mobilis ZM4 genome sequence
Start and end position in ZM4 | Location in coding or no-encoding region | Description of SNP (changes from ZM4 to 2032) |
|---|---|---|
995001–995001 | ZMO0976 | Insertion one “A,” and frame-shift and shorten ZMO0976 from 1023 to 708 bp (in both 2032 and 8b) |
1015532–1015532 | Promoter of ZMO0998 | C to T |
1263984–1265916 | ZMO1237 (ldhA) | 11 bp (CATGGGTCATA) deletion and 2881-bp duplicated Pgap-xylA xylB′ inserted into ZMO1237 (ldhA) 3′-end, with ZMO1237 shorten from 996 bp to 498 bp |
1265917–1266864 | ZMO1237 (ldhA) | Duplicated downstream and 3′ end of ZMO1237 sequence |
1266865–1272874 | ZMO1237 (ldhA) | 6010 bp Pgap-xylA xylB yiaB yiaA wecH tetA cassette inserted into ZMO1237 |
1272889–1272889 | ZMO1237 (ldhA) | G to A (S157S) (in both 2032 and 8b) |
1295154–1299678 | Promoter region of ZMO1268 and ZMO2012 | 4525 bp Tn5 cat Pgap-talB tktA inserted between ZMO1268 and ZMO2012 |
1299679–1299687 | Promoter region of ZMO1268 and ZMO2012 | Duplicated 9 bp ttcctagat (same sequence at 1295145–1295153) |
2007353–2007353 | ZMO1934 | C to T (E50K) (in both 2032 and 8b) |