Fig. 1

BODIPY and AC-202 stainings of lipid droplets in Chlamydomonas reinhardtii cells after nitrogen depletion. a Brightfield images of cells transferred to replete conditions (+Nitrogen, top) or media without nitrogen (−Nitrogen, bottom) after 48 h. b Fluorescence microscope images of cells transferred to replete conditions (+Nitrogen, top) or media without nitrogen (−Nitrogen, bottom) after 48 h and co-stained with BODIPY and AC-202. Single fluorescence channels and BODIPY + AC-202 fluorescence (Merge) are shown. c Normalized fluorescence profile plot of the nitrogen deprived cell in (b) showing the co-localization of BODIPY and AC-202 fluorescence. The x-axis represents the horizontal distance across the cell, and the y-axis the vertically averaged pixel intensity across the cell. d, e Total corrected cellular fluorescence was calculated for each fluorophore from cells grown under replete and nitrogen-starvation conditions after 24 h (d) and 48 h (e). Values are relative to the control condition (+Nitrogen). Averages shown, ± SE; ***P < 0.001, Students t test, n = 30; Scale bar, 5 µM