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Fig. 2 | Biotechnology for Biofuels

Fig. 2

From: AC-202, a highly effective fluorophore for the visualization of lipid droplets in green algae and diatoms

Fig. 2

BODPIPY and AC-202 stainings of lipid droplets in Chlamydomonas reinhardtii cells in the presence of a TOR inhibitor. a Brightfield images of cells treated with DMSO (top) or a TOR inhibitor (AZD8055, bottom) after 48 h. b Fluorescence microscope images of cells treated with DMSO (top) or a TOR inhibitor (AZD8055, bottom) for 48 h and co-stained with BODIPY and AC-202. Single fluorescence channels and BODIPY + AC-202 fluorescence (Merge) are shown. c Normalized fluorescence profile plot of the AZD8055-treated cell in (b) showing the co-localization of BODIPY and AC-202 fluorescence. The x-axis represents the horizontal distance across the cell, and the y-axis the vertically averaged pixel intensity across the cell. d, e Total corrected cellular fluorescence was calculated for each fluorophore from cells grown under control (DMSO) or TOR inhibition conditions (AZD8055) after 24 h (d) and 48 h (e). Values are relative to the control condition (DMSO). Averages shown, ± SE; **P < 0.001, ***P < 0.001, Students t-test, n = 30; Scale bar, 5 µM

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