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Fig. 4 | Biotechnology for Biofuels

Fig. 4

From: AC-202, a highly effective fluorophore for the visualization of lipid droplets in green algae and diatoms

Fig. 4

BODIPY and AC-202 staining of lipid droplets in Phaeodactylum tricornutum cells under nitrogen depletion. a Brightfield images of cells grown under replete conditions (+Nitrogen, top) or without nitrogen (−Nitrogen, bottom) after 72 h. b Fluorescence microscope images of cells grown under replete conditions (+Nitrogen, top) or without nitrogen (−Nitrogen, bottom) and co-stained with BODIPY and AC-202. c Normalized fluorescence profile plot of the cell in (b) showing the co-localization of BODIPY and AC-202 fluorescence. d Total corrected cellular fluorescence was calculated for each fluorophore from cells grown under replete and nitrogen-starvation conditions after 72 h. Values are relative to the control condition (+Nitrogen). Averages shown, ± SE; ***P < 0.001, Students t-test, n = 30; Scale bar, 5 µM

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