Fig. 1

Effect of temperature (a), pre-hydrolysis time (b), inoculum size (c), and pH (d) on SSCF of EDA-pretreated CS. The default conditions were 6% glucan loading, temperature 34 °C, pH = 4.8, inoculum size 2.5 g dry cell/L, 12-h pre-hydrolysis, and 150 rpm shaker speed. Ctec2 and Htec2 loadings were both 10 mg protein/g glucan. Error bars represent SD, n = 3, ns: not significant, *P < 0.05, **P < 0.01