Fig. 2

Effect of glucan loading (6, 7.5, and 9%) on SSCF of EDA-pretreated CS. a Glucose, xylose, and ethanol concentrations during SSCF. Error bars represent SD, n = 3. b–d Mass balance of SSCF with 6, 7.5, and 9% glucan loading. Other conditions were temperature 34 °C, pH = 4.8, inoculum size 2.5 g dry cell/L, 12-h pre-hydrolysis, and 150 rpm shaker speed. Ctec2 and Htec2 loadings were both 10 mg protein/g glucan