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Fig. 5 | Biotechnology for Biofuels

Fig. 5

From: Process analysis and optimization of simultaneous saccharification and co-fermentation of ethylenediamine-pretreated corn stover for ethanol production

Fig. 5

Performance of recycling SSCF process. a Determination of enzyme loading for cycle 2. Glucose, xylose, and ethanol concentrations after 48-h SSCF of cycle 2 with different enzyme loadings (0, 30, 50, 70, and 100%). Enzyme loading for cycle 1 is 20 mg/g glucan (Ctec2: Htec2 = 1:1) (100%). b Glucose, xylose, and ethanol concentrations before and after SSCF of each cycle. Enzyme loadings for cycles 2–5 were 50% of the cycle 1 and no enzyme loadings for the last step. Error bars represent SD, n = 2

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