Fig. 12

Measurements of total lignin content, S/G lignin monomer ratios, and total cell wall sugar release from the whole tillers of T0 stable transgenic switchgrass under the control of each of three green tissue-specific promoters. The whole tillers were harvested at the E5 growth stage with 5 representative lines of each construct being shown. Total lignin content and S/G lignin monomer ratios were measured using pyrolysis molecular beam mass spectrometry (py-MBMS) of cell wall residues (CWR). Sugar release efficiencies were determined by hot water pretreatment of CWR, followed by enzymatic hydrolysis. Bars represent the mean values of three independent replicates ± standard errors (vertical bars). Bars with asterisk are significantly different from controls at p ≤ 0.05 as calculated by t test. N.T., non-transgenic switchgrass