Fig. 2

Endogenous relative expression levels of the three switchgrass green tissue-specific genes in different tissues measured by real-time RT-PCR. RNA was extracted from switchgrass cv. ‘Alamo’ grown in the greenhouse at 28 °C under 16-h day/8-h night photoperiods (390 μE/m²/s) at the R1 growth stage. Relative quantification was performed using the standard curve method with PvUbi1 as the internal control gene. Bars represent the mean values of three independent replicates ± standard errors (vertical bars)