Switchgrass (Panicum virgatum L.) promoters for green tissue-specific expression of the MYB4 transcription factor for reduced-recalcitrance transgenic switchgrass

Table 1 Primer sequences used in this study

Gene	Primer	Primer Sequence (5′ > 3′)	Application
PvLhcb	PPvLhcb1-2F	CCCCGACCGATGCATCTACA	Promoter cloning
	PPvLhcb1-2R*	TGAGCCGAAGGAGGGTTGCT	Promoter cloning
	5Lhcb1-2-1F	CCTGTCACACACACAAGAGATGGC	Serial deletion
	5Lhcb1-2-2F	GTGAGAATATCTGGCGGCGAGC	Serial deletion
	Lhcb1qPCRF2	CTCGCCGACCACCTCACCGAT	qRT-PCR
	Lhcb1qPCRR1	CGGAAGACAATGAAGTTGCAGA	qRT-PCR
PvPEPC	PPvPEPCF	CACCGGCACTCTATGCTTAC	Promoter cloning
	PPvPEPCR*	CTAGCTAGCAGCTGCAGTTG	Promoter cloning
	5PEPC-1F	CGAGGAGCAGAAGAAGTCAC	Serial deletion
	5PEPC-2F	GCCACAGATTGACATGAAGTATCAC	Serial deletion
	5PEPC-3F	GCTCTAGTGTGAACGAAGTTCC	Serial deletion
	5PEPC-4F	CAGTAGCAGTACGGCAAGTC	Serial deletion
	PEPCqPCRF1	CTCATCCTCACCATGAAGGGT	qRT-PCR
	PEPCqPCRR1	TGCAGTTGAGGAGCGGAGCGC	qRT-PCR
PvPsbR	PPvPsbRF	GTTGGGCTGGTCTTGATCGT	Promoter cloning
	PPvPsbRR*	TTGCTGCTGCTTCGATTGCC	Promoter cloning
	PsbR-1F	ACGCACGGAATCACCTAGCAC	Serial deletion
	PsbR-2F	CAGCCCTGTATGCATGAAGTCC	Serial deletion
	PsbR-3F	GTTGGATGGCCTATCTTGTCCGG	Serial deletion
	PsbR-4F	GAGTCGATGCAGCATCCTCGATC	Serial deletion
	PsbR-5F	TCCCCAGAGGATAACGTTGCAG	Serial deletion
	PsbRqPCRF1	CCTTGTTTACAACACCAGCGCT	qRT-PCR
	PsbRqPCRR2	CCCAAGCTGATCTTACATG	qRT-PCR
PvUbi1	Ubi1-F	CAGCGAGGGCTCAATAATTCCA	qRT-PCR
	Ubi1-R	TCTGGCGGACTACAATATCCA	qRT-PCR
PvMYB4 ^a	MYB4-T-F	TCGGCATGCTCCTCGACTTC	qRT-PCR
	MYB4-T-R	ATCATAGGCGTCTCGCATATCT	qRT-PCR
PvMYB4 ^b	MYB4-E-F	AGGCCTCGAGATGAAGTGAAAC	qRT-PCR
	MYB4-E-R	AGCCCAACAAACAAACGAAATT	qRT-PCR

qRT-PCR, real-time RT-PCR
*Used as the reverse primers for cloning of the serial deletions and the promoter of each promoter
^aPrimers for the transgene
^bPrimers for the gene specific (endogenous)

ISSN: 2731-3654