Fig. 3

a Phylogenetic tree showing the placement of selected highly transcribed PGs. While 3737 reference genomes were used for alignment, only a subset of the aligned references was displayed. Blue diamonds indicate collapsed monophyletic clades with the number of reference genomes indicated in the brackets, black squares represent PGs from cultures amended with cellulose, and black triangles with xylan. Monophyletic clades of interest have been highlighted and bootstrap values (based on 100 iterations) are shown on internal nodes. The tree was midpoint-rooted. The scale bar indicates the evolutionary distance (substitution/site). PGs were color-coded by their enrichment culture, indigo for GZ-C-35, purple for SWH-C-35, red for GZ-X-35, green for SWH-X-35, and orange for SWH-C-55. b A heatmap showing the transcriptional level of the major energy conservation complexes and hydrogenases based on the TPM values of the corresponding genes. Time points in the order of day 5, mid-exponential growth phase, day 10 and day 15 were arranged as columns in the heatmap for each gene