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Fig. 3 | Biotechnology for Biofuels

Fig. 3

From: CIPK9 is involved in seed oil regulation in Brassica napus L. and Arabidopsis thaliana (L.) Heynh.

Fig. 3

Transgenic rapeseed plants overexpressing ProBnNapin:BnCIPK9T178D (BnCIPK9-OE) and analyses of seed oil content, seed size, and seed weight. a T3 generation of dried seeds of four independent ProBnNapin:BnCIPK9T178D overexpressing transgenic lines (OE-1, OE-2, OE-3, OE-4) and J572 (wild type, WT), used as the control. Bars correspond to 500 µm. b Expression levels of BnCIPK9 in the seeds of transgenic rapeseed lines as assessed by qRT-PCR. Bars represent mean ± standard deviation (SD) (three technical replicates). c Total seed oil contents of transgenic rapeseed plants (T2), and J572 (WT) used as the control. Bars indicate the SDs of different replicates. Asterisks indicate significant differences (P < 0.05) between transgenic and control (J572) plants. d Dry seed weight (15 seeds) of transgenic and J572 rapeseed plants. Error bars indicate the SD (n ≥ 5)

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