Fig. 4
From: CIPK9 is involved in seed oil regulation in Brassica napus L. and Arabidopsis thaliana (L.) Heynh.

Promoter and expression pattern of BnCIPk9 in seedlings, in the presence and absence of sugars. a Composition of putative cis-acting elements in the BnCIPK9 promoter (BnCIPK9 promoter1, 3050 bp from the original fragment; BnCIPK9 promoter2, 3372 bp from the original fragment). b Activity of BnCIPK9 promoter1 in 7-day-old seedlings in the presence and absence of sugars. The transgenic fusion including 3050 bp (BnCIPK9 promoter1) of the 5′-upstream regulatory region of the BnCIPK9, fused to the GUS reporter gene used to generate the transgenic lines (BnCIPK9 promoter1:GUS). c Activity of the promoter2 of BnCIPK9 in 7-day-old seedlings in the presence or absence of sugars. The transgenic fusion included 3372 bp (BnCIPK9 promoter2) of the 5′-upstream regulatory region of BnCIPK9, fused to the GUS reporter gene used to generate the transgenic lines (BnCIPK9 promoter2:GUS). Different sugar sources [1 or 3% glucose (Glu), or sucrose (Suc)] were used; mannitol (Mlt) was used as a control for osmotic stress. Data are shown as means of the relative GUS activity of promoter1 and promoter2 of BnCIPK9± standard deviation (SD) (n = 3). The Student’s t test was performed to evaluate the significance of differences between means at *P < 0.05; **P < 0.01