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Table 2 Strains and plasmids used in this study

From: Engineering and systems-level analysis of Pseudomonas chlororaphis for production of phenazine-1-carboxamide using glycerol as the cost-effective carbon source

Strains and plasmids Relevant genotype or description Source or reference
Strains E. coli DH5α E. coli FФ80lacZΔM15 Δ(lacZYA-argF) M15 U169 recA1 endA1 hsdR17 (rk mk) phoA supE44 thi−1 gyrA96 relA1 Invitrogen
E. coli S17-1 (λpir) res pro mod+ integrated copy of RP4, mob+, used for incorporating constructs into P. chlororaphis [58]
P. chlororaphis HT66 P. chlororaphis HT66 Wild-type, Apr Spr China Center for type culture collection
HT66L lon in-frame deletion mutant of HT66 This study
HT66LS parS in-frame deletion mutant of HT66L This study
HT66LR parR in-frame deletion mutant of HT66L This study
HT66LSP psrA in-frame deletion mutant of HT66LS This study
Plasmids pK18mobsacB Broad-host-range gene replacement vector; sacB, Kanr [59]
pK18-lon pK18mobsacB containing lon flanking region This study
pK18-parS pK18mobsacB containing parS flanking region This study
pK18-parR pK18mobsacB containing parR flanking region This study
pK18-psrA pK18mobsacB containing psrA flanking region This study