Table 2 Strains and plasmids used in this study
Strains and plasmids | Relevant genotype or description | Source or reference |
|---|---|---|
Strains E. coli DH5α | E. coli F−Ф80lacZΔM15 Δ(lacZYA-argF) M15 U169 recA1 endA1 hsdR17 (rk− mk−) phoA supE44 thi−1 gyrA96 relA1 | Invitrogen |
E. coli S17-1 (λpir) | res− pro mod+ integrated copy of RP4, mob+, used for incorporating constructs into P. chlororaphis | [58] |
P. chlororaphis HT66 | P. chlororaphis HT66 Wild-type, Apr Spr | China Center for type culture collection |
HT66L | lon in-frame deletion mutant of HT66 | This study |
HT66LS | parS in-frame deletion mutant of HT66L | This study |
HT66LR | parR in-frame deletion mutant of HT66L | This study |
HT66LSP | psrA in-frame deletion mutant of HT66LS | This study |
Plasmids pK18mobsacB | Broad-host-range gene replacement vector; sacB, Kanr | [59] |
pK18-lon | pK18mobsacB containing lon flanking region | This study |
pK18-parS | pK18mobsacB containing parS flanking region | This study |
pK18-parR | pK18mobsacB containing parR flanking region | This study |
pK18-psrA | pK18mobsacB containing psrA flanking region | This study |