Fig. 2

Stability and optimal pH and temperature of BGL-3, using 0.1% pNPG as substrate. Lines indicate the optimum temperature and pH values for enzyme activity; bars show its stability after 72 h in a range of temperatures from 30 to 70 °C or in a range of pH from 2 to 10. For temperature stability assays, the enzyme was incubated with sodium acetate buffer pH 4.0, 100 mM. For pH stability tests, the enzyme was incubated at the appropriate pH in Britton–Robinson buffer, 100 mM at 4 °C. All reactions were performed in triplicate