Table 1 Purification of the BGL-3 secreted in Mandels medium with glucose as carbon source by T. amestolkiae cultures
BGL-3 purification | ||||
|---|---|---|---|---|
Step | Total protein (mg) | Total activity (U) | Specific activity (U/mg) | Yield (%) |
Crude extracts | 1.2 | 25.9 | 21.5 | 100 |
HiTrap Capto adhere | 0.7 | 10.8 | 42.4 | 41.7 |