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Table 2 Kinetic parameters of BGL-3 from T. amestolkiae against different substrates. All reactions were performed in triplicate

From: The β-glucosidase secreted by Talaromyces amestolkiae under carbon starvation: a versatile catalyst for biofuel production from plant and algal biomass

Substrate Km (mM) Vmax (U/mg) kcat (s−1) kcat/Km (mM−1 s−1)
pNPG 0.14 ± 0.1 381.1 ± 4.1 1359.4 9710.2
oNPG 0.14 ± 0.1 142.1 ± 3.6 506.9 3425.2
Cellobiose 0.48 ± 0.1 447.1 ± 7.0 1594.6 3308.4
Cellotriose 0.80 ± 0.1 275.7 ± 4.2 983.3 1216.9
Cellotetraose 0.35 ± 0.1 373.0 ± 5.0 1330.4 3779.5
Cellopentaose 0.32 ± 0.1 408.6 ± 6.9 1457.2 4442.7
Cellohexaose 0.57 ± 0.1 374.1 ± 3.0 1334.2 2304.4
Laminaribiose 6.34 ± 0.2 290.8 ± 5.3 1037.1 163.4
Gentiobiose 9.77 ± 0.3 299.2 ± 3.8 1067.2 109.1
Laminarin from L. digitata 1.21 ± 0.1 142.4 ± 1.0 253.9 211.5
Laminarin from L. hyperborea 1.1 ± 0.2 139.0 ± 2.0 247.9 225.3