Table 2 Kinetic parameters of BGL-3 from T. amestolkiae against different substrates. All reactions were performed in triplicate
Substrate | Km (mM) | Vmax (U/mg) | kcat (s−1) | kcat/Km (mM−1 s−1) |
|---|---|---|---|---|
pNPG | 0.14 ± 0.1 | 381.1 ± 4.1 | 1359.4 | 9710.2 |
oNPG | 0.14 ± 0.1 | 142.1 ± 3.6 | 506.9 | 3425.2 |
Cellobiose | 0.48 ± 0.1 | 447.1 ± 7.0 | 1594.6 | 3308.4 |
Cellotriose | 0.80 ± 0.1 | 275.7 ± 4.2 | 983.3 | 1216.9 |
Cellotetraose | 0.35 ± 0.1 | 373.0 ± 5.0 | 1330.4 | 3779.5 |
Cellopentaose | 0.32 ± 0.1 | 408.6 ± 6.9 | 1457.2 | 4442.7 |
Cellohexaose | 0.57 ± 0.1 | 374.1 ± 3.0 | 1334.2 | 2304.4 |
Laminaribiose | 6.34 ± 0.2 | 290.8 ± 5.3 | 1037.1 | 163.4 |
Gentiobiose | 9.77 ± 0.3 | 299.2 ± 3.8 | 1067.2 | 109.1 |
Laminarin from L. digitata | 1.21 ± 0.1 | 142.4 ± 1.0 | 253.9 | 211.5 |
Laminarin from L. hyperborea | 1.1 ± 0.2 | 139.0 ± 2.0 | 247.9 | 225.3 |