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Fig. 3 | Biotechnology for Biofuels

Fig. 3

From: An endogenous microRNA (miRNA1166.1) can regulate photobio-H2 production in eukaryotic green alga Chlamydomonas reinhardtii

Fig. 3

Selection of high H2 production transgenic algae. The Y-axis (H2 concentration) indicates the H2 content in 1 mL gas sample detected by gas chromatography (GC), which was calculated from the H2 peak area. 0 untreated controls; HS1h heat shock treatment for 1 h; R1h, R3h, and R5h recovery at 22 °C for 1, 3, and 5 h after heat treatment, respectively. Since H2 detection of each sample takes 7–8 min, biological replicates were performed for each transgenic algal strain to avoid large treatment time differences. Biological replicates are shown in Fig. 6, Additional file 3: Figure S2, and Additional file 4: Figure S3

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