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Fig. 4 | Biotechnology for Biofuels

Fig. 4

From: A downstream box fusion allows stable accumulation of a bacterial cellulase in Chlamydomonas reinhardtii chloroplasts

Fig. 4

TetC-Cel6A accumulation during growth of transplastomic C. reinhardtii strains. a Growth curve of C. reinhardtii wild-type and transformed strains harboring the cel6A, NPTII-cel6A, and TetC-cel6A genes regulated by the 16SP and atpA 5′UTR, grown in TAP media. A representative of triplicate growth curves for each cell line is shown. b Evaluation of the TetC-Cel6A protein accumulation throughout the cellular growth phases. The wild-type cell line was used as a negative control. All lanes contained 50 µg total soluble protein (TSP) extracted from either the wild-type or the TetC-cel6A strain

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