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Fig. 5 | Biotechnology for Biofuels

Fig. 5

From: A downstream box fusion allows stable accumulation of a bacterial cellulase in Chlamydomonas reinhardtii chloroplasts

Fig. 5

Effect of nitrogen starvation on the accumulation of TetC-Cel6A. a Immunoblots showing the TetC-Cel6A protein accumulation after 5, 24, and 48 h of resuspension in N-replete (top blot) or N-deplete (bottom blot) minimal media. Exposure time was 2 min for both blots. All lanes contained 10 µg total soluble protein. Control lane contained 0.01 µg purified Cel6A protein. The TetC-Cel6A protein accumulation was quantified in two biological replicates by comparing the relative blot density of bands to those of a purified Cel6A control. bd Several parameters monitored in the TetC-Cel6A expressing cells grown under N-replete or N-deplete media for 5, 24, and 48 h. Data are the average of two biological replicates per treatment and time point. Error bars are the standard error of the mean. Statistical analysis were performed by Student’s t test, *p < 0.05 and ns = not significant

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