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Table 1 Strains and plasmids used in this study

From: Enhanced biological fixation of methane for microbial lipid production by recombinant Methylomicrobium buryatense

Strain/plasmid Description References
Strains
 E. coli TOP10 F–mcrA Δ (mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ (ara leu) 7697 galU galK rpsL (StrR) endA1 nupG Invitrogen
 E. coli S17-1 λpir Donor strain. TpR SmR recA, thi, pro, hsd (R-M+) RP4: 2-Tc:Mu: Km Tn7 λpir [27]
 M. buryatense 5GB1 Moderately haloalkaliphilic methanotroph [17]
 M. buryatense 5GB1 ∆glgA1glgA2 M. buryatense 5GB1 ∆glgA1glgA2 [17]
 AP18 M. buryatense 5GB1C [17] ∆glgA1glgA2sps This study
Plasmids
 pAWP93 pCM433kanT containing flanks to knock out repA-repB locus of native plasmid. [17]
 pAB2 Kanamycin-resistant variant of pCM433 containing flanks to knock out sps This study