Table 1 Strains and plasmids used in this study

Strains

 E. coli TOP10

F–mcrA Δ (mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ (ara leu) 7697 galU galK rpsL (Str^R) endA1 nupG

Invitrogen

 E. coli S17-1 λpir

Donor strain. Tp^R Sm^R recA, thi, pro, hsd (R^-M⁺) RP4: 2-Tc:Mu: Km Tn7 λpir

[27]

 M. buryatense 5GB1

Moderately haloalkaliphilic methanotroph

[17]

 M. buryatense 5GB1 ∆glgA1 ∆glgA2

M. buryatense 5GB1 ∆glgA1 ∆glgA2

[17]

 AP18

M. buryatense 5GB1C [17] ∆glgA1 ∆glgA2 ∆sps

This study

Plasmids

 pAWP93

pCM433kanT containing flanks to knock out repA-repB locus of native plasmid.

[17]

 pAB2

Kanamycin-resistant variant of pCM433 containing flanks to knock out sps

This study