Table 2 Primers used in this study
Primer name | Sequence (5′–3′) | Description |
|---|---|---|
AP186_pCM433kanT_fwd1 | ATGTGCAGGTTGTCGGTGTC | For amplifying the backbone of the KanR version of the sucrose counterselection plasmid pCM433 [17] |
AP187_pCM433kanT_rev1 | TGGTAACTGTCAGACCAAGTTTACTC | |
AP110_spsKO_UP_Fwd | ATTGGTACCATGGATGCATATGCTGCAGCTACGCTGCTCTAAATACCTTG | For amplifying flanks to knock out sps (MaGE locus tag MBURv2_130613) using plasmid pAB2 |
AP111_spsKO_UP_Rev | TCTGCTCTCAGTCACGATCGTACTCACTTCAGCTG | |
AP112_spsKO_DWN_Fwd | GAAGTGAGTACGATCGTGACTGAGAGCAGAATTATGACTG | |
AP113_spsKO_DWN_Rev | AGTGAGCTCACCGGTTAACACGCGTACGTACCTTCGGCCAAATACAACGA |