Fig. 4

Fermentation time profiles for enzymatic hydrolysates obtained from AFEX™, StEx (washed solids), StEx (unwashed solids), StEx (whole slurry) and StEx (C₅-liquor) sugarcane bagasse and cane leaf matter. Fermentations were performed using S. cerevisiae 424A (LHN-ST) with an initial inoculum of 0.96 g CDW L⁻¹ at 30 °C, pH 5.5, and a shaking speed of 150 rpm for 120 h. All enzymatic hydrolysates were supplemented with 0.25% (w/w) corn steep liquor prior to fermentation. Square—glucose (g L⁻¹), Triangle—xylose (g L⁻¹), Circle—ethanol (g L⁻¹), Diamond—OD_600nm