Fig. 3
From: High-oleate yeast oil without polyunsaturated fatty acids
Heterologous Δ9 fatty acid desaturase expression in Y. lipolytica. Of the 7 heterologous Δ9 fatty acid desaturase genes individually transformed into a Δole1 strain, 5 gave rise to transformants able to grow without monounsaturated fatty acid supplementation. Two isolates of each gene as well as duplicates of the wild-type strain were grown in nitrogen-limited media for 96 h and cells were analyzed by gas chromatography to obtain the lipid profile shown