Fig. 4
From: High-oleate yeast oil without polyunsaturated fatty acids
High-oleate strain engineering. A simplified pathway for fatty acid and TAG synthesis in Y. lipolytica is used to illustrate the gene deletions (Ylole1, Ylsct1, Ylfad2) and heterologous gene expressions (PgOLE1, AaSCT1, rELO2, RtDGA1, CpDGA2) incorporated into the high-oleate strain NS993 (a). The indicated strains were grown in triplicate in nitrogen-limited media for 96Â h. Cells were analyzed by gas chromatography and fluorescence assay to obtain the lipid profile (b) and lipid content (c) shown. Fatty acid levels are reported as the percent fraction of total C16 and C18 fatty acids quantitated. Total lipid content is reported as FL/OD normalized to wild-type. Values shown are the average of triplicate cultures and error bars represent standard deviation. FAS fatty acid synthase, GPAT: glycerol-3-phosphate acyltransferase, LPAT lysophosphatidic acid acyltransferase, PAP phosphatidate phosphatase, DGAT diacylglycerol acyltransferase, TAG triacylglycerol