Fig. 3

Balance of intracellular and extracellular biotin during glutamic acid fermentation by C. glutamicum in CSH. a Biotin balance inside and outside the cells. b Biotin content in cells. CSH at 15% solids content (w/w) was used here. Fermentation was carried out in flasks as described in the Methods section without penicillin induction. Mean values were presented with error bars representing at least two standard deviations