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Table 1 Parameters and results of chemostat culture of R. toruloides

From: Systems analysis of phosphate-limitation-induced lipid accumulation by the oleaginous yeast Rhodosporidium toruloides

Culture code F3 (Pi-replete) P0 (Pi-limited)
Feeding nutrients
 Glucose (g/l) 27 27
 PO43− (mM) 10.14 0.11
 C/P molar ratio 63 2164
 Dilution rate (h−1) 0.3 0.085
Effluent nutrients
 Glucose (g/l) 22.3 ± 0.6a 22.3 ± 0.6a
 PO43− (mM) 7.35 ± 0.25a ND
Production parameters
 Cell mass (g/l) 0.30 ± 0.07a 0.83 ± 0.06a
 Lipid content (%) 7.3 ± 2.3a 43.9 ± 0.5a
 YX,S (g cell mass per g glucose) 0.06 ± 0.01a 0.18 ± 0.01a
 YP,S (mg oil per g glucose) 4.5 ± 1.4a 88.3 ± 5.8a
 YX,Pi (g cell mass per g PO43−) 1.1 ± 0.2a 79.4 ± 6.7a
 YP,Pi (g oil per g PO43−) 0.08 ± 0.02a 34.8 ± 2.1a
  1. F3, medium with abundant phosphate with dilution rate of 0.3 h−1; P0, medium with trivial amount of phosphate with dilution rate of 0.085 h−1; ND, not detectable
  2. aAverage ± s.d. of three biological samples