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Table 1 Parameters and results of chemostat culture of R. toruloides

From: Systems analysis of phosphate-limitation-induced lipid accumulation by the oleaginous yeast Rhodosporidium toruloides

Culture code

F3 (Pi-replete)

P0 (Pi-limited)

Feeding nutrients

 Glucose (g/l)

27

27

 PO43− (mM)

10.14

0.11

 C/P molar ratio

63

2164

 Dilution rate (h−1)

0.3

0.085

Effluent nutrients

 Glucose (g/l)

22.3 ± 0.6a

22.3 ± 0.6a

 PO43− (mM)

7.35 ± 0.25a

ND

Production parameters

 Cell mass (g/l)

0.30 ± 0.07a

0.83 ± 0.06a

 Lipid content (%)

7.3 ± 2.3a

43.9 ± 0.5a

 YX,S (g cell mass per g glucose)

0.06 ± 0.01a

0.18 ± 0.01a

 YP,S (mg oil per g glucose)

4.5 ± 1.4a

88.3 ± 5.8a

 YX,Pi (g cell mass per g PO43−)

1.1 ± 0.2a

79.4 ± 6.7a

 YP,Pi (g oil per g PO43−)

0.08 ± 0.02a

34.8 ± 2.1a

  1. F3, medium with abundant phosphate with dilution rate of 0.3 h−1; P0, medium with trivial amount of phosphate with dilution rate of 0.085 h−1; ND, not detectable
  2. aAverage ± s.d. of three biological samples