Table 1 Microbial strains used in the present study
Strain | Parental strain | Relevant genotype/expressed gene | Source of references |
|---|---|---|---|
E. coli DH5 | Φ80dlacZΔm15, recA1, endA1, gyrA96, thi-1, hsdR17 (rk−, mk+), supE44, relA1, deoR, Δ(lacZYA-argF) U169 | Invitrogen | |
YLp | Po1d | MATA, leu2-270, ura3-302, xpr2-322, ∆pox1-6 | [27] |
YLpW | YLp | LEU2, URA3 | [32] |
YLpO | YLp | pTEF-SCD1-LEU2, pEXP-DGA1-URA3 | This investigation |
YLpL | YLp | LEU2, pTEF-LIP2-URA3 | This investigation |
YLx | YLp | ΔDGAT1-2, ΔLRO1, ΔFAD2 | [22] |
YLxW | YLx | LEU2, URA3 | This investigation |
YLxR | YLx | LEU2, URA3, pTEF-CpFAH12-HygroR | This investigation |
CYLp | YLp | pEXP-BGL1, pHTEF-BGL2, pHTEF-EG1, pEXP-EG2, pHTEF-CBH1, pHTEF-CBH2-LEU2 | This investigation |
CYLpW | CYLp | URA3 | This investigation |
CYLpO | CYLp | pTEF-SCD1, pEXP-DGA1-URA3 | This investigation |
CYLpL | CYLp | pTEF-LIP2-URA3 | This investigation |
CYLx | YLx | pEXP-BGL1, pHTEF-BGL2, pHTEF-EG1, pEXP-EG2, pHTEF-NcCBH1-URA3, pHTEF-TrCBH2-LEU2 | This investigation |
CYLxR | CYLx | pTEF-CpFAH12-HygroR | This investigation |