Developing cellulolytic Yarrowia lipolytica as a platform for the production of valuable products in consolidated bioprocessing of cellulose

Table 5 Comparison of lipid and biomass yield of recombinant Y. lipolytica strains grown on 80 g/L glucose or 72.8 g/L cellulose (C/N ratio ≈ 30:1) in shake flasks for 5 days

	Y_X/S (g DCW/g)	Y_FAME/S (g/g)	Y_FAME/X (g/g DCW)	P_FAME (g/L/h)
MM-glucose
YLpW	0.26 ± 0.02	0.030 ± 0.004	0.12 ± 0.02	0.016 ± 0.002
YLpO	0.28 ± 0.01	0.092 ± 0.005	0.30 ± 0.02	0.073 ± 0.003
CYLpO	0.27 ± 0.02	0.088 ± 0.003	0.29 ± 0.01	0.070 ± 0.004
MM-cellulose^a
YLpO + 10 FPU/g	0.26 ± 0.02	0.058 ± 0.004	0.22 ± 0.01	0.024 ± 0.004
YLpO + 20 FPU/g	0.27 ± 0.00	0.091 ± 0.003	0.31 ± 0.03	0.073 ± 0.003
CYLpO	0.25 ± 0.03	0.036 ± 0.002	0.14 ± 0.02	0.005 ± 0.001
CYLpO + 10 FPU/g	0.26 ± 0.01	0.085 ± 0.004	0.30 ± 0.02	0.069 ± 0.002

Results were calculated from at least three biological replicates and are given as the mean values ± standard deviation
^aYields were calculated from the glucose consumption deduced from the cellulose degradation

ISSN: 2731-3654