Fig. 1

Tracking surface accessibility of lignocellulosic components in α-cellulose and Avicel using FTCM-depletion assay (a, b) and total composition analysis using the NREL/TP-510-42618 method (c). a α-Cellulose and Avicel were incubated with the GC3a probe (0.5 µg/µL; for crystalline cellulose detection), CC17 probe (0.5 µg/µL; for non-crystalline cellulose detection, OC15 probe (0.5 µg/µL; for xylan detection) and the CC27 probe (0.5 µg/µL; for mannan detection) for 1 h at room temperature under tumbling agitation. The fluorescence values were converted to bound probes (µmol/g of biomass) using the standard curves (Additional file 6). Green, cherry, orange and cyan colors represent the GC3a, CC17, OC15 and CC27 probe detection, respectively. b The addition of the binding of GC3a and CC17, from (a), represents the total cellulose (GC3a + CC17) and the addition of the binding of OC15 and CC27, from (a), represents the total hemicelluloses (OC15 + CC27). c Total composition analysis of α-cellulose and Avicel using the standard NREL/TP-510-42618 method