Table 1 Identification of relevant proteins expressed only on kraft lignin (KL) or vanillic acid (VL) that can assist in lignin degradation
Uniprot entry | Gene locus tag | Protein names | LFQ intensity KL | Razor + unique peptides KL | Sequence coverage (%) | Mol. weight (kDa) | Intensity |
|---|---|---|---|---|---|---|---|
Relevant protein expressed only on kraft lignin (KL) | |||||||
A0A1E3LHD6 | A9762_20370 | Tryptophan 2,3-dioxygenase | 22.9781 | 2 | 12.3 | 36.655 | 0.00043 |
A0A1E3LB56 | A9762_07750 | Benzoyl-CoA oxygenase subunit B | 28.4307 | 15 | 41.7 | 54.265 | 2.06E−87 |
A0A1E3LET3 | A9762_23815 | Acriflavine resistance protein | 22.5124 | 1 | 1.2 | 112.36 | 0.00162 |
A0A1E3LL12 | A9762_14780 | Glycine betaine ABC transporter substrate-binding protein | 23.8554 | 2 | 6.2 | 36.204 | 0.00036 |
A0A1E3LI04 | A9762_22630 | Enoyl-CoA hydratase | 23.5827 | 2 | 13.3 | 28.399 | 1.38E−14 |
A0A1E3LPU3 | A9762_13345 | Pyruvate ferredoxin oxidoreductase | 17.3225 | 3 | 4 | 129.05 | 0.00016 |
A0A1E3LLU4 | A9762_13050 | Carboxyvinyl-carboxyphosphonate phosphorylmutase | 24.4057 | 2 | 14.3 | 31.557 | 0.00039 |
A0A1E3LBB9 | A9762_07755 | Benzoyl-CoA oxygenase/reductase, BoxA protein | 23.9233 | 2 | 5 | 45.826 | 5.25E−07 |
A0A1E3LGI4 | A9762_03990 | SAM-dependent methyltransferase | 24.6086 | 2 | 9.6 | 31.525 | 1.73E−06 |
A0A1E3LDW7 | A9762_25245 | (2Fe–2S)-binding protein | 24.4339 | 2 | 24.5 | 20.242 | 2.06E−06 |
A0A1E3LNU5 | A9762_10215 | LysR family transcriptional regulator | 23.5144 | 2 | 8.4 | 33.65 | 4.15E−07 |
A0A1E3LEP7 | A9762_23880 | Phenylacetic acid degradation protein | 24.5346 | 3 | 12.4 | 39.494 | 4.34E−14 |
A0A1E3LJ38 | A9762_17050 | ABC transporter | 26.2152 | 5 | 18.3 | 32.972 | 3.46E−34 |
A0A1E3LB77 | A9762_07880 | ABC transporter ATP-binding protein | 24.5787 | 2 | 9.7 | 25.723 | 2.69E−12 |
A0A1E3LF42 | A9762_23860 | 1,2-Phenylacetyl-CoA epoxidase subunit A (monooxygenase) | 25.5084 | 4 | 14 | 37.739 | 4.64E−20 |
A0A1E3LGK2 | A9762_23865 | 1,2-Phenylacetyl-CoA epoxidase subunit B (monooxygenase) | 24.5067 | 2 | 20.4 | 11.224 | 6.77E−06 |
A0A1E3LHG4 | A9762_23220 | Formyl-CoA:oxalate CoA transferase | 26.7341 | 5 | 17.8 | 45.737 | 4.76E−34 |
A0A1E3LNE1 | A9762_10935 | Salicylate hydroxylase | 22.5412 | 1 | 3.1 | 41.287 | 0.00183 |
A0A1E3LF93 | A9762_23590 | Ligand-gated channel protein | 24.4315 | 2 | 3.4 | 81.344 | 8.95E−05 |
A0A1E3LHJ6 | A9762_19845 | NADPH:quinone reductase | 23.3346 | 1 | 3.1 | 31.317 | 0.00029 |
A0A1E3LIQ8 | A9762_17970 | Glycolate oxidase subunit GlcE | 24.8966 | 2 | 7.3 | 40.542 | 3.71E−10 |
A0A1E3LEZ8 | A9762_23215 | 2-Hydroxyhepta-2,4-diene-1,7-dioate isomerase | 24.1273 | 2 | 14.8 | 27.8 | 1.39E−06 |
Relevant protein expressed only on vanillic acid (VA) | |||||||
A0A1E3LRS2 | A9762_00545 | Alkene reductase | 26.4047 | 7 | 29.1 | 39.612 | 3.91E−37 |
A0A1E3LLI9 | A9762_02605 | Alpha/beta hydrolase | 25.8742 | 4 | 23.2 | 30.983 | 3.92E−11 |
A0A1E3LLX9 | A9762_03340 | Tol-pal system-associated acyl-CoA thioesterase | 24.2249 | 2 | 14.4 | 17.548 | 2.38E−05 |
A0A1E3LDT8 | A9762_25265 | Acetyltransferase | 24.2249 | 2 | 14.4 | 17.548 | 2.38E−05 |
A0A1E3LPL0 | A9762_01420 | Glutathione S-transferase | 24.4839 | 2 | 15.3 | 24.629 | 2.35E−07 |
A0A1E3LCR5 | A9762_26030 | Aminomethyltransferase | 25.197 | 2 | 11 | 34.276 | 8.63E−05 |
A0A1E3LPI9 | A9762_13065 | Methyltransferase | 22.991 | 2 | 7.7 | 29.85 | 1.53E−06 |
A0A1E3LEN5 | A9762_06460 | Rieske (2Fe–2S) protein | 25.0064 | 1 | 6.5 | 43.065 | 1.25E−08 |
A0A1E3LHR2 | A9762_19260 | Glycine/betaine ABC transporter permease | 24.0824 | 1 | 4.9 | 25.659 | 0.0001153 |