Fig. 3

Production of 1-octanol with S. cerevisiae. Strain RPY21 expressing mutated FAS^R1834K as well as CAR and Sfp from a high-copy plasmid (black), was grown for 72 h in buffered YPD medium. For reference, analogous combinations of wild-type FAS, CAR, and Sfp (red) as well as mutated FAS^R1834K with empty vector instead of CAR and Sfp are shown (blue). Error bars reflect the standard deviations from three biological replicates. OD₆₀₀ (a) and extracellular 1-octanol concentrations (b) were analyzed at different time points. c Final octanoic acid concentration in the extracellular culture medium after 72 h. FAS^RK means FAS^R1834K