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Fig. 3 | Biotechnology for Biofuels

Fig. 3

From: An engineered fatty acid synthase combined with a carboxylic acid reductase enables de novo production of 1-octanol in Saccharomyces cerevisiae

Fig. 3

Production of 1-octanol with S. cerevisiae. Strain RPY21 expressing mutated FASR1834K as well as CAR and Sfp from a high-copy plasmid (black), was grown for 72 h in buffered YPD medium. For reference, analogous combinations of wild-type FAS, CAR, and Sfp (red) as well as mutated FASR1834K with empty vector instead of CAR and Sfp are shown (blue). Error bars reflect the standard deviations from three biological replicates. OD600 (a) and extracellular 1-octanol concentrations (b) were analyzed at different time points. c Final octanoic acid concentration in the extracellular culture medium after 72 h. FASRK means FASR1834K

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