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Fig. 6 | Biotechnology for Biofuels

Fig. 6

From: An engineered fatty acid synthase combined with a carboxylic acid reductase enables de novo production of 1-octanol in Saccharomyces cerevisiae

Fig. 6

Increase in 1-octanol production in a yeast strain by additional overexpression of heterologous Ahr. Strain RPY21/FASR1834K expressing CAR and Sfp together with Ahr from E. coli from a high-copy plasmid (black), was grown for 72 h in buffered YPD medium. For comparison, analogous combinations of wild-type FAS with CAR, Sfp, and Ahr (red) as well as mutated FASR1834K with empty vector instead of Ahr are shown (blue). Error bars indicate the standard deviation from three biological replicates. a OD600 and 1-octanol b were analyzed at different time points. c Final octanoic acid concentration after 72 h. FASRK means FASR1834K

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