Fig. 8

In situ extraction of 1-octanol and octanoic acid in a two-phase fermentation of yeast. Strain RPY21/FAS^R1834K overexpressing CAR and Sfp together with Ahr (a) and control strain RPY21/FAS^R1834K (b) were grown for 72 h, in buffered YPD medium overlaid with (+) and without (−) 20% of dodecane. Error bars reflect the standard deviations from two biological replicates. After 72 h, the final OD₆₀₀ (·) and the final concentrations of 1-octanol and octanoic acid were analyzed. FAS^RK means FAS^R1834K. The gray areas indicate concentrations of 1-octanol or octanoic acid, respectively, calculated on the basis of the volume of the aqueous phase of the culture, but based on the amounts measured in the dodecane phase. The white-colored areas indicate concentrations measured directly in the aqueous phase