Table 1 List of plasmids used in this study

pRS315-FAS1-Wt

CEN6ARS4, AmpR, LEU2, p _FAS1 -^ScFAS1-Wt-t _FAS1

[53]

pRS315-FAS1-RK

CEN6ARS4, AmpR, LEU2, p _FAS1 -^ScFAS1^R1834K-t _FAS1

[53]

pRS313-FAS2-Wt

CEN6ARS4, AmpR, HIS3, p _FAS2 -^ScFAS2-Wt-t _FAS2

[53]

pRS62H

2µ, hphNT1, AmpR, truncated HXT7 promotor (p ^−1-−392_HXT7 ) and FBA1 terminator

[68]

pRS62 N

2µ, natNT2, AmpR, shortened HXT7 promotor (p ^−1-−392_HXT7 ) and CYC1 terminator

[68]

pRS62H-CAR-Sfp

pRS62H; p ^−1-−392 _HXT7 -^MmCAR-t _FBA ; p _PGK1 -^BsSfp-t _PGM2

This study

pRS62 N-Ahr

pRS62N; p ^−1-−392 _HXT7 -^EcAhr-t _CYC1

This study