An engineered fatty acid synthase combined with a carboxylic acid reductase enables de novo production of 1-octanol in Saccharomyces cerevisiae

Table 2 Relevant primers for plasmid construction

Primer name	Sequence 5′–3′	Amplicon
SHP35_pPGK1_for	AACCCTGGCGTTACCCAACTTAATCGCCTTGCAG CATGTTTGCAAAAAGAACAAAACTG	PGK1 promotor
CBP160_pPGK1_rev	TGTTTTATATTTGTTGTAAAAAGTAGATAATTAC	PGK1 promotor
CBP35_tPGM2_for	AACGAATGATTTACTAATGGC	PGM2 terminator
SHP36_tPGM2_rev	GAAATCGGCAAAATCCCTTATAAATCAAAAGAAT AGACAAAAAACTCGGGGTAGGTAAT	PGM2 terminator
SHP54_Ahr_for	CAAAAACAAAAAGTTTTTTTAATTTTAATCAAAAA ATGTCGATGATAAAAAGCTATGCC	Ahr ORF
SHP53_Ahr_rev	ATGTAAGCGTGACATAACTAATTACATGACTCGA GTCAAAAATCGGCTTTCAACAC	Ahr ORF
DR_Faa2	GGAAGAATGCAGGTTACAAAAAACGGATAAGAA CAAACTTGTTTCGAAATGTACTTATGACGATTTGG AACACATTCAAACTAGAAAAAACTTTGATGTA	Donor-DNA fragment for FAA2 deletion
CC_FAA2-rev	CGTAAGGTTTCAAAATCTTCGATCATTTATCTTTC ACTGCGGAG	Amplification of a CRISPR-Cas9 plasmid pRCCN for deletion of FAA2, reverse
CC_FAA2-fw	GAAGATTTTGAAACCTTACGGTTTTAGAGCTAGAA ATAGCAAGTTAAAATAAGG	Amplification of a CRISPR-Cas9 plasmid pRCCN for deletion of FAA2, forward

The abbreviations within the primer names were used as follows: forward primer (fw) and reverse primer (rev)

ISSN: 2731-3654