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Table 2 Conserved degrees of the amino acid residues located in the active pockets and catalytic channels of XYL10C-ΔN and other 49 GH10 counterparts

From: Insight into the functional roles of Glu175 in the hyperthermostable xylanase XYL10C-ΔN through structural analysis and site-saturation mutagenesis

Amino acidsa

Location

Conserved degreeb

Statistics

272 Tyr (Asn)

Active pocket

9

15× Y, 10× G, 6× S, 3× D, 3× R, 3× T, 3× W, 2× N, 2× Q, 1× A,1× E,1× F

307 Glu (Glu)

Active pocket

7

15× Q, 7× E, 7× S, 5× G, 2× A, 2× N, 2× R, 2× V, 1× D, 1× H, 1× L, 1× M, 1× P, 1× T

384 Thr (Ala)

Active pocket and catalytic channel

5

25× T, 14× V, 3× S, 2× F, 1× A, 1× E, 1× Q, 1× W

173 Gln (Ala)

Active pocket

2

35× H, 11× Y, 2× Q, 1× A, 1× D

269 Asn (Gly)

Active pocket

2

42× N, 3× G, 3× S, 1× A

230 Gln (Ser)

Active pocket

1

44× R, 2× Q, 1× K, 1× S, 1× V

175 Glu (Gln)

Active pocket and catalytic channel

1

44× Q, 4× E, 1× G, 1× P

300 Glu (Glu)

Active pocket

1

42× Q, 8× E

135 Lys (Lys)

Active pocket and catalytic channel

1

49× K, 1× D

168 His (His)

Active pocket and catalytic channel

1

49× H, 1× D

372 Trp (Trp)

Active pocket and catalytic channel

1

50× W

218 Asn (Asn)

Active pocket

1

49× N

  1. aPosition and amino acid of XYL10C-ΔN (the corresponding residue in XylE)
  2. bThe lower values correspond to higher conservation