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Table 2 Conserved degrees of the amino acid residues located in the active pockets and catalytic channels of XYL10C-ΔN and other 49 GH10 counterparts

From: Insight into the functional roles of Glu175 in the hyperthermostable xylanase XYL10C-ΔN through structural analysis and site-saturation mutagenesis

Amino acidsa Location Conserved degreeb Statistics
272 Tyr (Asn) Active pocket 9 15× Y, 10× G, 6× S, 3× D, 3× R, 3× T, 3× W, 2× N, 2× Q, 1× A,1× E,1× F
307 Glu (Glu) Active pocket 7 15× Q, 7× E, 7× S, 5× G, 2× A, 2× N, 2× R, 2× V, 1× D, 1× H, 1× L, 1× M, 1× P, 1× T
384 Thr (Ala) Active pocket and catalytic channel 5 25× T, 14× V, 3× S, 2× F, 1× A, 1× E, 1× Q, 1× W
173 Gln (Ala) Active pocket 2 35× H, 11× Y, 2× Q, 1× A, 1× D
269 Asn (Gly) Active pocket 2 42× N, 3× G, 3× S, 1× A
230 Gln (Ser) Active pocket 1 44× R, 2× Q, 1× K, 1× S, 1× V
175 Glu (Gln) Active pocket and catalytic channel 1 44× Q, 4× E, 1× G, 1× P
300 Glu (Glu) Active pocket 1 42× Q, 8× E
135 Lys (Lys) Active pocket and catalytic channel 1 49× K, 1× D
168 His (His) Active pocket and catalytic channel 1 49× H, 1× D
372 Trp (Trp) Active pocket and catalytic channel 1 50× W
218 Asn (Asn) Active pocket 1 49× N
  1. aPosition and amino acid of XYL10C-ΔN (the corresponding residue in XylE)
  2. bThe lower values correspond to higher conservation