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Fig. 1 | Biotechnology for Biofuels

Fig. 1

From: Biomimetic strategy for constructing Clostridium thermocellum cellulosomal operons in Bacillus subtilis

Fig. 1

Schematic diagrams of a biomimetic cellulosome and a biomimetic operon. a Schematic representation of a biomimetic cellulosome and assembly of cellulosomal subunits on the cell surface. The cell-surface anchor (sdbA, shown in blue) is attached on the cell membrane. The scaffolding protein (CipA, shown in yellow) is composed primarily of nine copies of specific Type I cohesin and Type III carbohydrate-binding modules (CBM). b Construction of biomimetic polycistronic operons, Type I and Type II in pGETS 118 vector, restriction enzymes were given in red color. c The confirmation of gene assembly pattern by EcoRI Restriction digestion

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