Fig. 5

Identification of CtPMO1 reaction products oxidized by Br₂ with celloheptaose as substrate using MALDI-TOF–MS. Reaction products upon incubation of 0.1% celloheptaose with CtPMO1 in 10 mM HAc-NH₄Ac (pH 5.0) and 1 mM ascorbate at 50 °C for 48 h. Reaction products (a) and reaction products oxidized by Br₂ (b). C1-oxidized oligosaccharides (m/z + 16), C4- or C6-oxidized oligosaccharides (m/z − 2), C4- and C1-oxidized oligosaccharides (m/z + 14), C6- and C1-oxidized oligosaccharides (m/z + 30), and C1-, C6- and C4-oxidized oligosaccharides (m/z + 28)