Fig. 6
From: Regioselectivity of oxidation by a polysaccharide monooxygenase from Chaetomium thermophilum
Identification of the mutated CtPMO1 soluble reaction products with PASC as substrate using TLC. Soluble reaction products upon incubation of 0.5% PASC with the mutated CtPMO1 enzymes (Y27A, H64A, H157A and Y206A) in 10 mM HAc-NH4Ac (pH 5.0) and 1 mM ascorbate at 50 °C for 48 h. M, standard cellulo-oligosaccharides (G1–G7). CK, samples upon incubation of 0.5% PASC in 10 mM HAc-NH4Ac (pH 5.0) and 1 mM ascorbate at 50 °C for 48 h with inactive CtPMO1 treated at 100 °C for 30 min