Fig. 7
From: Regioselectivity of oxidation by a polysaccharide monooxygenase from Chaetomium thermophilum
Identification of the mutated CtPMO1 soluble reaction products with PASC as substrate using MALDI-TOF–MS. Soluble reaction products upon incubation of 0.5% PASC with the mutated CtPMO1 enzymes Y27A (a), H64A (b), H157A (c) and Y206A (d) in 10 mM HAc-NH4Ac (pH 5.0) and 1 mM ascorbate at 50 °C for 48 h. C1-oxidized oligosaccharides (aldonic acid, m/z + 16) and C4- or C6-oxidized oligosaccharides (C4-ketoaldose or C6-hexodialdose, m/z − 2)