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Fig. 7 | Biotechnology for Biofuels

Fig. 7

From: Regioselectivity of oxidation by a polysaccharide monooxygenase from Chaetomium thermophilum

Fig. 7

Identification of the mutated CtPMO1 soluble reaction products with PASC as substrate using MALDI-TOF–MS. Soluble reaction products upon incubation of 0.5% PASC with the mutated CtPMO1 enzymes Y27A (a), H64A (b), H157A (c) and Y206A (d) in 10 mM HAc-NH4Ac (pH 5.0) and 1 mM ascorbate at 50 °C for 48 h. C1-oxidized oligosaccharides (aldonic acid, m/z + 16) and C4- or C6-oxidized oligosaccharides (C4-ketoaldose or C6-hexodialdose, m/z − 2)

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