Fig. 9
From: Regioselectivity of oxidation by a polysaccharide monooxygenase from Chaetomium thermophilum
Mutated CtPMO1 insoluble reaction products oxidized by Br2 with PASC as substrate using MALDI-TOF–MS. Insoluble reaction products (residual PASC) upon incubation of 0.5% PASC with mutated CtPMO1 Y27A (a), H64A (b), H157A (c) and Y206A (d) in 10 mM HAc-NH4Ac (pH 5.0) and 1 mM ascorbate at 50 °C for 48 h. C1-oxidized oligosaccharides (m/z + 16), C4-oxidized oligosaccharides (m/z + 14), and C6-oxidized oligosaccharides (m/z + 30)