Fig. 2

Change in pH of pretreated wheat straw slurry during 24 h of incubation. a Effect of incubation temperature and b effect of DM content in the absence of enzymes. Steam-pretreated wheat straw was diluted to DM contents of 1, 2, 5, or 10% (wt/wt), the pH was adjusted to 5.3, and the samples incubated at 40, 45, 50, or 60 °C. c, d Show the effect of enzyme addition to pretreated wheat straw slurry at 10% DM incubated at 50 °C. The data shown in (d) are the result of re-adjusting the pH to 5.0–5.1 and continuing incubation for an additional 24 h. The values given are the average of 3–12 replicates, and the error bars represent the standard deviation. No enzyme no addition of enzymes, Cat catalase; CTec3: Cellic^® CTec3; Cell: Celluclast 1.5L; BG: β-glucosidase; BSA: bovine serum albumin; AA9: TaAA9A