Fig. 3
Acetic acid release and pH in steam-pretreated wheat straw slurry adjusted to pH 5.3 during incubation at 50 °C. a Acetic acid released during the saccharification of 10% DM wheat straw slurry. The CTec3 loading was 2.8 (D1) or 8.4 (D2) mg total protein/g cellulose. The catalase loading was 0.08 mg protein/g DM (approximately 11,000 U/ml slurry). b Addition of catalase reduces the decrease in pH during 24 and 48 h incubation of 2% DM, and 10% DM incubated for 24 and 48 h. c Effect of added H2O2 (to a concentration of 2.9 and 29 mM) on the pH of 10% DM wheat straw slurry after 24 h incubation at 50 °C under ambient air and reduced-oxygen conditions. The values given are the average of duplicate samples, and the error bars represent the standard deviation. Cat catalase, CTec3 Cellic CTec3