Fig. 6

Production of CO₂ during incubation of pretreated wheat straw adjusted to pH 5.3. a Effect of temperature, and b DM content, during incubation for 24 h. DM contents of 1, 2, 5, and 10%, at 40, 45, 50, and 60 °C. The values given are the average of four replicates, and the error bars represent the standard deviation. c Dosage-dependent production of CO₂ during the incubation of 10% DM pretreated wheat straw slurry with H₂O₂ at 50 °C for 24 h under ambient atmosphere (grey bars) or reduced-oxygen conditions (green bars). The CO₂ produced in samples without the addition of H₂O₂ has been subtracted. d Effect of enzymes on CO₂ production during the first 24 h at 50 °C after initial pH adjustment to 5.3 and e during the following 24 h after pH re-adjustment to 5. No enzyme no addition of enzymes, Cat catalase, CTec3 CelliC CTec3, BSA bovine serum albumin, AA9 TaAA9