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Fig. 2 | Biotechnology for Biofuels

Fig. 2

From: The synchronous TAG production with the growth by the expression of chloroplast transit peptide-fused ScPDAT in Chlamydomonas reinhardtii

Fig. 2

The construction and effects of exogenous ScPDAT. a The map of pChlamy–ScPDAT plasmid. PUCori: (high-copy replication and growth in E. coli, 673 bp), Hsp70A–RbcS2 (a hybrid constitutive promoter, 495 bp), Int-1 Rbc S2 (maintains the high expression of ScPDAT, 144 bp), β2-tubulin (drives the expression of Aph7 gene, 312 bp), Hygromycin (selection of C. reinhardtii, 1310 bp), Ampicillin (selection of the plasmid in E. coli, 860 bp), Pbla (expression of the ampicillin resistance gene, 51 bp). b Western blot of ScPDAT in Scpdat, the growth curve of wild type and Scpdat. Cells sampled during a 5-day culture cycle and grown in TAP medium. Each type of algae takes three bottles a day and does not put them back. Shown are mean values and SD; n = 3. Assay conditions are described in “Methods”. c The chlorophyll fluorescence of wild type and Scpdat. Shown are mean values and SD; n = 3. Assay conditions are described in “Methods

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