The synchronous TAG production with the growth by the expression of chloroplast transit peptide-fused ScPDAT in Chlamydomonas reinhardtii

Table 1 A functional study of PDAT derived from different species

Gene	Origin	Research	Conclusion	References
CrPDAT	Chlamydomonas reinhardtii	Utilizing RNA-Seq insert mutants Artificial microRNA silencing of PDAT	Demonstrating the relevance of the transacylation pathway CrPDAT possesses acyl hydrolase activities, mediated membrane lipid turnover and degradation	[13] [10]
ScPDAT	Saccharomyces cerevisiae	Lacking the predicted membrane-spanning region of ScPDAT expressed in Pichia pastoris	ScPDAT can catalyze a number of transacylation reactions at a low rate	[11]
AtPDAT	Arabidopsis	Exploring role of enzymes in TAG synthesis by RNA interference Over-expression of AtPDAT in microsomal preparations of roots and leaves Coexpression of PDAT1 with oleosin Disruption of SDP1 TAG lipase or PXA1 severely decreases FA turnover, leading to increases in leaf TAG accumulation	The velocity of AtPDAT dependent on acyl composition Enhancing fatty acid synthesis and diverting fatty acids from membrane lipids to triacylglycerol Over-expression of PDAT1 enhances the turnover of FAs in leaf lipids	[9] [12] [14] [15]
LuPDAT	Linum usitatissimum L.	Over-expression of LuPDAT genes in yeast and Arabidopsis	Certain PDATs have the unique ability to efficiently channel ALA into TAG	[16]
MiPDAT	Myrmecia incisa Reisigl	Over-expression of MiPDAT and increase transcription levels in M. incisa	The mechanism is discussed that MiPDAT in this microalgal uses PC to yield TAG	[1]

ISSN: 2731-3654